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Srivastava Lab

Runs FindTopFeatures on the embedded Seurat object and returns the updated MatisseObject. Selects the most accessible peaks for downstream LSI / UMAP.

Usage

# S3 method for class 'MatisseObject'
FindTopFeatures(object, assay = NULL, min.cutoff = "q5", verbose = TRUE, ...)

Arguments

object

A MatisseObject.

assay

Name of assay to use

min.cutoff

Cutoff for feature to be included in the VariableFeatures for the object. This can be a percentile specified as 'q' followed by the minimum percentile, for example 'q5' to set the top 95\ as the VariableFeatures for the object. Alternatively, this can be an integer specifying the minimum number of counts for the feature to be included in the set of VariableFeatures. For example, setting to 10 will include features with >10 total counts in the set of VariableFeatures. If NULL, include all features in VariableFeatures. If NA, VariableFeatures will not be altered, and only the feature metadata will be updated with the total counts and percentile rank for each feature.

verbose

Display messages

...

Additional arguments forwarded to FindTopFeatures (e.g. min.cutoff).

Value

The updated MatisseObject with top ATAC features flagged.